Construct: shRNA TRCN0000107152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015558.3-685s1c1
DNA Barcode:
CCAGTCGTCCATCGCCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SS18L1 (26039)
Gene Description:
SS18L1 subunit of BAF chromatin remodeling complex
Transcript:
RefSeq NM_015558.3 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCGTCCATCGCCATGAT
Hairpin Sequence:
5'-CCGG-CCAGTCGTCCATCGCCATGAT-CTCGAG-ATCATGGCGATGGACGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCGTCCATCGCCATGATCTCGAGATCATGGCGATGGACGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCGTCCATCGCCATGATCTCGAGATCATGGCGATGGACGACTGG-3'

Other clones with same target sequence:

(none)