Construct: shRNA TRCN0000107189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015675.1-226s1c1
DNA Barcode:
GTCGGCCAAGTTGATGAATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GADD45B (4616)
Gene Description:
growth arrest and DNA damage inducible beta
Transcript:
RefSeq NM_015675.1 (NON-CURRENT)
Match location:
Position 226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGCCAAGTTGATGAATGT
Hairpin Sequence:
5'-CCGG-GTCGGCCAAGTTGATGAATGT-CTCGAG-ACATTCATCAACTTGGCCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGCCAAGTTGATGAATGTCTCGAGACATTCATCAACTTGGCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGCCAAGTTGATGAATGTCTCGAGACATTCATCAACTTGGCCGAC-3'

Other clones with same target sequence:

(none)