Construct: shRNA TRCN0000107215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004394.1-1332s1c1
DNA Barcode:
CCAGATAAGAAACCTGCCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAP (1611)
Gene Description:
death associated protein
Transcript:
RefSeq NM_004394.1 (NON-CURRENT)
Match location:
Position 1332 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATAAGAAACCTGCCCTT
Hairpin Sequence:
5'-CCGG-CCAGATAAGAAACCTGCCCTT-CTCGAG-AAGGGCAGGTTTCTTATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATAAGAAACCTGCCCTTCTCGAGAAGGGCAGGTTTCTTATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATAAGAAACCTGCCCTTCTCGAGAAGGGCAGGTTTCTTATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000333268