Construct: shRNA TRCN0000107257

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203349.2-1760s1c1
DNA Barcode:
CCCAGCGAGATACCTCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHC4 (399694)
Gene Description:
SHC adaptor protein 4
Transcript:
RefSeq NM_203349.2 (NON-CURRENT)
Match location:
Position 1760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107257)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCGAGATACCTCATTAT
Hairpin Sequence:
5'-CCGG-CCCAGCGAGATACCTCATTAT-CTCGAG-ATAATGAGGTATCTCGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCGAGATACCTCATTATCTCGAGATAATGAGGTATCTCGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCGAGATACCTCATTATCTCGAGATAATGAGGTATCTCGCTGGG-3'

Other clones with same target sequence:

(none)