Construct: shRNA TRCN0000107267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006106.2-1373s1c1
DNA Barcode:
CAGGTGATACTATCAACCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YAP1 (10413)
Gene Description:
Yes associated protein 1
Transcript:
RefSeq NM_006106.2 (NON-CURRENT)
Match location:
Position 1373 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGTGATACTATCAACCAAA
Hairpin Sequence:
5'-CCGG-CAGGTGATACTATCAACCAAA-CTCGAG-TTTGGTTGATAGTATCACCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGTGATACTATCAACCAAACTCGAGTTTGGTTGATAGTATCACCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGTGATACTATCAACCAAACTCGAGTTTGGTTGATAGTATCACCTG-3'

Other clones with same target sequence:

  1. TRCN0000310615