Construct: shRNA TRCN0000107396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025169.1-590s1c1
DNA Barcode:
GCAATTGTGTTACCACGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZKSCAN7 (55888)
Gene Description:
zinc finger with KRAB and SCAN domains 7
Transcript:
RefSeq NM_025169.1 (NON-CURRENT)
Match location:
Position 590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTGTGTTACCACGAGAT
Hairpin Sequence:
5'-CCGG-GCAATTGTGTTACCACGAGAT-CTCGAG-ATCTCGTGGTAACACAATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTGTGTTACCACGAGATCTCGAGATCTCGTGGTAACACAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTGTGTTACCACGAGATCTCGAGATCTCGTGGTAACACAATTGC-3'

Other clones with same target sequence:

(none)