Construct: shRNA TRCN0000107400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138456.3-1068s1c1
DNA Barcode:
CCCAGGATTTCACAGTCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BATF2 (116071)
Gene Description:
basic leucine zipper ATF-like transcription factor 2
Transcript:
RefSeq NM_138456.3 (NON-CURRENT)
Match location:
Position 1068 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGATTTCACAGTCGAAA
Hairpin Sequence:
5'-CCGG-CCCAGGATTTCACAGTCGAAA-CTCGAG-TTTCGACTGTGAAATCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGATTTCACAGTCGAAACTCGAGTTTCGACTGTGAAATCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGATTTCACAGTCGAAACTCGAGTTTCGACTGTGAAATCCTGGG-3'

Other clones with same target sequence:

(none)