Construct: shRNA TRCN0000107446

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018197.2-2142s1c1
DNA Barcode:
CTGGCAATCAAACTTTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFP64 (55734)
Gene Description:
ZFP64 zinc finger protein
Transcript:
RefSeq NM_018197.2 (NON-CURRENT)
Match location:
Position 2142 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107446)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCAATCAAACTTTCATTA
Hairpin Sequence:
5'-CCGG-CTGGCAATCAAACTTTCATTA-CTCGAG-TAATGAAAGTTTGATTGCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCAATCAAACTTTCATTACTCGAGTAATGAAAGTTTGATTGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCAATCAAACTTTCATTACTCGAGTAATGAAAGTTTGATTGCCAG-3'

Other clones with same target sequence:

(none)