Construct: shRNA TRCN0000107530

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004304.2-1960s1c1
DNA Barcode:
GCTGCTCAAGAAACAAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF740 (283337)
Gene Description:
zinc finger protein 740
Transcript:
RefSeq NM_001004304.2 (NON-CURRENT)
Match location:
Position 1960 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107530)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCAAGAAACAAAGGAA
Hairpin Sequence:
5'-CCGG-GCTGCTCAAGAAACAAAGGAA-CTCGAG-TTCCTTTGTTTCTTGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCAAGAAACAAAGGAACTCGAGTTCCTTTGTTTCTTGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCAAGAAACAAAGGAACTCGAGTTCCTTTGTTTCTTGAGCAGC-3'

Other clones with same target sequence:

(none)