Construct: shRNA TRCN0000107571

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178864.2-1603s1c1
DNA Barcode:
CCTTCCCAGATCCACTAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPAS4 (266743)
Gene Description:
neuronal PAS domain protein 4
Transcript:
RefSeq NM_178864.2 (NON-CURRENT)
Match location:
Position 1603 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107571)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCCAGATCCACTAACTA
Hairpin Sequence:
5'-CCGG-CCTTCCCAGATCCACTAACTA-CTCGAG-TAGTTAGTGGATCTGGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCCAGATCCACTAACTACTCGAGTAGTTAGTGGATCTGGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCCAGATCCACTAACTACTCGAGTAGTTAGTGGATCTGGGAAGG-3'

Other clones with same target sequence:

(none)