Construct: shRNA TRCN0000107586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152629.2-2374s1c1
DNA Barcode:
CGGGCATTACAGTGTATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLIS3 (169792)
Gene Description:
GLIS family zinc finger 3
Transcript:
RefSeq NM_152629.2 (NON-CURRENT)
Match location:
Position 2374 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCATTACAGTGTATGATT
Hairpin Sequence:
5'-CCGG-CGGGCATTACAGTGTATGATT-CTCGAG-AATCATACACTGTAATGCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCATTACAGTGTATGATTCTCGAGAATCATACACTGTAATGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCATTACAGTGTATGATTCTCGAGAATCATACACTGTAATGCCCG-3'

Other clones with same target sequence:

(none)