Construct: shRNA TRCN0000107775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_058999.9-1477s1c1
DNA Barcode:
CCGGAGAGACAATGGAGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF787 (126208)
Gene Description:
zinc finger protein 787
Transcript:
RefSeq XM_058999.9 (NON-CURRENT)
Match location:
Position 1477 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGAGACAATGGAGAGAA
Hairpin Sequence:
5'-CCGG-CCGGAGAGACAATGGAGAGAA-CTCGAG-TTCTCTCCATTGTCTCTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGAGACAATGGAGAGAACTCGAGTTCTCTCCATTGTCTCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGAGACAATGGAGAGAACTCGAGTTCTCTCCATTGTCTCTCCGG-3'

Other clones with same target sequence:

(none)