Construct: shRNA TRCN0000107795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199358.1-1957s1c1
DNA Barcode:
CGTGCCATCTTTAATGTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXD4L3 (387054)
Gene Description:
forkhead box D4-like 3
Transcript:
RefSeq NM_199358.1 (NON-CURRENT)
Match location:
Position 1957 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCCATCTTTAATGTTAAA
Hairpin Sequence:
5'-CCGG-CGTGCCATCTTTAATGTTAAA-CTCGAG-TTTAACATTAAAGATGGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCCATCTTTAATGTTAAACTCGAGTTTAACATTAAAGATGGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCCATCTTTAATGTTAAACTCGAGTTTAACATTAAAGATGGCACG-3'

Other clones with same target sequence:

(none)