Construct: shRNA TRCN0000107943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006101.1-1068s1c1
DNA Barcode:
GCCATTCTTGACCAGAAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDC80 (10403)
Gene Description:
NDC80 kinetochore complex component
Transcript:
RefSeq NM_006101.1 (NON-CURRENT)
Match location:
Position 1068 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTCTTGACCAGAAATTA
Hairpin Sequence:
5'-CCGG-GCCATTCTTGACCAGAAATTA-CTCGAG-TAATTTCTGGTCAAGAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTCTTGACCAGAAATTACTCGAGTAATTTCTGGTCAAGAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTCTTGACCAGAAATTACTCGAGTAATTTCTGGTCAAGAATGGC-3'

Other clones with same target sequence:

(none)