Construct: shRNA TRCN0000107967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_379850.1-397s1c1
DNA Barcode:
GCTCGTTCTCACACGTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC402524 (402524)
Gene Description:
similar to Zinc finger protein 58 (Zfp-58) (Zinc finger protein Mfg-1)
Transcript:
RefSeq XM_379850.1 (NON-CURRENT)
Match location:
Position 397 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTTCTCACACGTCATTA
Hairpin Sequence:
5'-CCGG-GCTCGTTCTCACACGTCATTA-CTCGAG-TAATGACGTGTGAGAACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTTCTCACACGTCATTACTCGAGTAATGACGTGTGAGAACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTTCTCACACGTCATTACTCGAGTAATGACGTGTGAGAACGAGC-3'

Other clones with same target sequence:

(none)