Construct: shRNA TRCN0000108002

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004314.2-2184s1c1
DNA Barcode:
GCCTTCGAATACATGGACTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF788P (388507)
Gene Description:
zinc finger family member 788, pseudogene
Transcript:
RefSeq NM_001004314.2 (NON-CURRENT)
Match location:
Position 2184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108002)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCGAATACATGGACTAA
Hairpin Sequence:
5'-CCGG-GCCTTCGAATACATGGACTAA-CTCGAG-TTAGTCCATGTATTCGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCGAATACATGGACTAACTCGAGTTAGTCCATGTATTCGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCGAATACATGGACTAACTCGAGTTAGTCCATGTATTCGAAGGC-3'

Other clones with same target sequence:

(none)