Construct: shRNA TRCN0000108119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007125.3-4225s1c1
DNA Barcode:
CTGGGCATGAATACAGTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTY (7404)
Gene Description:
ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Transcript:
RefSeq NM_007125.3 (NON-CURRENT)
Match location:
Position 4225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGGCATGAATACAGTACAA
Hairpin Sequence:
5'-CCGG-CTGGGCATGAATACAGTACAA-CTCGAG-TTGTACTGTATTCATGCCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGGCATGAATACAGTACAACTCGAGTTGTACTGTATTCATGCCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGGCATGAATACAGTACAACTCGAGTTGTACTGTATTCATGCCCAG-3'

Other clones with same target sequence:

(none)