Construct: shRNA TRCN0000108177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017637.4-1428s1c1
DNA Barcode:
CCCTGTCATAGCAAGTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BNC2 (54796)
Gene Description:
basonuclin 2
Transcript:
RefSeq NM_017637.4 (NON-CURRENT)
Match location:
Position 1428 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTCATAGCAAGTACAAA
Hairpin Sequence:
5'-CCGG-CCCTGTCATAGCAAGTACAAA-CTCGAG-TTTGTACTTGCTATGACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTCATAGCAAGTACAAACTCGAGTTTGTACTTGCTATGACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTCATAGCAAGTACAAACTCGAGTTTGTACTTGCTATGACAGGG-3'

Other clones with same target sequence:

(none)