Construct: shRNA TRCN0000108182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_098940.6-1607s1c1
DNA Barcode:
CGGGAGAGAAACCATACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF782 (158431)
Gene Description:
zinc finger protein 782
Transcript:
RefSeq XM_098940.6 (NON-CURRENT)
Match location:
Position 1607 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGAGAAACCATACAAAT
Hairpin Sequence:
5'-CCGG-CGGGAGAGAAACCATACAAAT-CTCGAG-ATTTGTATGGTTTCTCTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGAGAAACCATACAAATCTCGAGATTTGTATGGTTTCTCTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGAGAAACCATACAAATCTCGAGATTTGTATGGTTTCTCTCCCG-3'

Other clones with same target sequence:

(none)