Construct: shRNA TRCN0000108199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374430.1-2833s1c1
DNA Barcode:
GACCTCCCTACGAACAACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMIZ2 (83637)
Gene Description:
zinc finger MIZ-type containing 2
Transcript:
RefSeq XM_374430.1 (NON-CURRENT)
Match location:
Position 2833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTCCCTACGAACAACAAT
Hairpin Sequence:
5'-CCGG-GACCTCCCTACGAACAACAAT-CTCGAG-ATTGTTGTTCGTAGGGAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTCCCTACGAACAACAATCTCGAGATTGTTGTTCGTAGGGAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTCCCTACGAACAACAATCTCGAGATTGTTGTTCGTAGGGAGGTC-3'

Other clones with same target sequence:

  1. TRCN0000300961