Construct: shRNA TRCN0000108263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178012.3-1174s1c1
DNA Barcode:
CCTGAAGATGTCGGCCACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBB2B (347733)
Gene Description:
tubulin beta 2B class IIb
Transcript:
RefSeq NM_178012.3 (NON-CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGATGTCGGCCACCTT
Hairpin Sequence:
5'-CCGG-CCTGAAGATGTCGGCCACCTT-CTCGAG-AAGGTGGCCGACATCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGATGTCGGCCACCTTCTCGAGAAGGTGGCCGACATCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGATGTCGGCCACCTTCTCGAGAAGGTGGCCGACATCTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000300955