Construct: shRNA TRCN0000108322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001856.2-1783s1c1
DNA Barcode:
CCTGAAGGGTTCCAGAACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL16A1 (1307)
Gene Description:
collagen type XVI alpha 1 chain
Transcript:
RefSeq NM_001856.2 (NON-CURRENT)
Match location:
Position 1783 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGGGTTCCAGAACTTT
Hairpin Sequence:
5'-CCGG-CCTGAAGGGTTCCAGAACTTT-CTCGAG-AAAGTTCTGGAACCCTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGGGTTCCAGAACTTTCTCGAGAAAGTTCTGGAACCCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGGGTTCCAGAACTTTCTCGAGAAAGTTCTGGAACCCTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000300062