Construct: shRNA TRCN0000108325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020445.3-1605s1c1
DNA Barcode:
GCTGACAAATACAATTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTR3B (57180)
Gene Description:
actin related protein 3B
Transcript:
RefSeq NM_020445.3 (NON-CURRENT)
Match location:
Position 1605 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACAAATACAATTCTGAA
Hairpin Sequence:
5'-CCGG-GCTGACAAATACAATTCTGAA-CTCGAG-TTCAGAATTGTATTTGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACAAATACAATTCTGAACTCGAGTTCAGAATTGTATTTGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACAAATACAATTCTGAACTCGAGTTCAGAATTGTATTTGTCAGC-3'

Other clones with same target sequence:

(none)