Construct: shRNA TRCN0000108365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017992.1-1239s1c1
DNA Barcode:
GCTTCCATATTAGTGGGCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTBL2 (345651)
Gene Description:
actin beta like 2
Transcript:
RefSeq NM_001017992.1 (NON-CURRENT)
Match location:
Position 1239 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCATATTAGTGGGCTTA
Hairpin Sequence:
5'-CCGG-GCTTCCATATTAGTGGGCTTA-CTCGAG-TAAGCCCACTAATATGGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCATATTAGTGGGCTTACTCGAGTAAGCCCACTAATATGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCATATTAGTGGGCTTACTCGAGTAAGCCCACTAATATGGAAGC-3'

Other clones with same target sequence:

(none)