Construct: shRNA TRCN0000108379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006091.1-901s1c1
DNA Barcode:
CTACGAGATCAGCACTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CORO2B (10391)
Gene Description:
coronin 2B
Transcript:
RefSeq NM_006091.1 (NON-CURRENT)
Match location:
Position 901 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACGAGATCAGCACTGAGAA
Hairpin Sequence:
5'-CCGG-CTACGAGATCAGCACTGAGAA-CTCGAG-TTCTCAGTGCTGATCTCGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACGAGATCAGCACTGAGAACTCGAGTTCTCAGTGCTGATCTCGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACGAGATCAGCACTGAGAACTCGAGTTCTCAGTGCTGATCTCGTAG-3'

Other clones with same target sequence:

(none)