Construct: shRNA TRCN0000108385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004520.1-2128s1c1
DNA Barcode:
GCCATTTGAAAGTTTGGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF2A (3796)
Gene Description:
kinesin family member 2A
Transcript:
RefSeq NM_004520.1 (NON-CURRENT)
Match location:
Position 2128 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTTGAAAGTTTGGAATT
Hairpin Sequence:
5'-CCGG-GCCATTTGAAAGTTTGGAATT-CTCGAG-AATTCCAAACTTTCAAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTTGAAAGTTTGGAATTCTCGAGAATTCCAAACTTTCAAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTTGAAAGTTTGGAATTCTCGAGAATTCCAAACTTTCAAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000300438