Construct: shRNA TRCN0000108411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004370.4-5453s1c1
DNA Barcode:
GCAGAAATATAGGATCACTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL12A1 (1303)
Gene Description:
collagen type XII alpha 1 chain
Transcript:
RefSeq NM_004370.4 (NON-CURRENT)
Match location:
Position 5453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAATATAGGATCACTTA
Hairpin Sequence:
5'-CCGG-GCAGAAATATAGGATCACTTA-CTCGAG-TAAGTGATCCTATATTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAATATAGGATCACTTACTCGAGTAAGTGATCCTATATTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAATATAGGATCACTTACTCGAGTAAGTGATCCTATATTTCTGC-3'

Other clones with same target sequence:

(none)