Construct: shRNA TRCN0000108437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003980.3-784s1c1
DNA Barcode:
CGGTCAGTTTCCACCATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP7 (9053)
Gene Description:
microtubule associated protein 7
Transcript:
RefSeq NM_003980.3 (NON-CURRENT)
Match location:
Position 784 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCAGTTTCCACCATGAAT
Hairpin Sequence:
5'-CCGG-CGGTCAGTTTCCACCATGAAT-CTCGAG-ATTCATGGTGGAAACTGACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCAGTTTCCACCATGAATCTCGAGATTCATGGTGGAAACTGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCAGTTTCCACCATGAATCTCGAGATTCATGGTGGAAACTGACCG-3'

Other clones with same target sequence:

  1. TRCN0000300437