Construct: shRNA TRCN0000108454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016718.1-401s1c1
DNA Barcode:
GCCACCATCTTGGTCTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ninj2 (29862)
Gene Description:
ninjurin 2
Transcript:
RefSeq NM_016718.1 (NON-CURRENT)
Match location:
Position 401 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCATCTTGGTCTTCATA
Hairpin Sequence:
5'-CCGG-GCCACCATCTTGGTCTTCATA-CTCGAG-TATGAAGACCAAGATGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCATCTTGGTCTTCATACTCGAGTATGAAGACCAAGATGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCATCTTGGTCTTCATACTCGAGTATGAAGACCAAGATGGTGGC-3'

Other clones with same target sequence:

(none)