Construct: shRNA TRCN0000108481

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008516.1-1025s1c1
DNA Barcode:
CCAGAACAACTTTACAAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrn1 (16979)
Gene Description:
leucine rich repeat protein 1, neuronal
Transcript:
RefSeq NM_008516.1 (NON-CURRENT)
Match location:
Position 1025 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108481)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAACAACTTTACAAACAT
Hairpin Sequence:
5'-CCGG-CCAGAACAACTTTACAAACAT-CTCGAG-ATGTTTGTAAAGTTGTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAACAACTTTACAAACATCTCGAGATGTTTGTAAAGTTGTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAACAACTTTACAAACATCTCGAGATGTTTGTAAAGTTGTTCTGG-3'

Other clones with same target sequence:

(none)