Construct: shRNA TRCN0000108505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177152.4-3727s1c1
DNA Barcode:
CCGGGAATAACTTTCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrig3 (320398)
Gene Description:
leucine-rich repeats and immunoglobulin-like domains 3
Transcript:
RefSeq NM_177152.4 (NON-CURRENT)
Match location:
Position 3727 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAATAACTTTCATATAA
Hairpin Sequence:
5'-CCGG-CCGGGAATAACTTTCATATAA-CTCGAG-TTATATGAAAGTTATTCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAATAACTTTCATATAACTCGAGTTATATGAAAGTTATTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAATAACTTTCATATAACTCGAGTTATATGAAAGTTATTCCCGG-3'

Other clones with same target sequence:

(none)