Construct: shRNA TRCN0000108510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011983.1-1178s1c1
DNA Barcode:
CATTCTCCATTTGCTTCTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Homer2 (26557)
Gene Description:
homer scaffolding protein 2
Transcript:
RefSeq NM_011983.1 (NON-CURRENT)
Match location:
Position 1178 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCTCCATTTGCTTCTGTA
Hairpin Sequence:
5'-CCGG-CATTCTCCATTTGCTTCTGTA-CTCGAG-TACAGAAGCAAATGGAGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCTCCATTTGCTTCTGTACTCGAGTACAGAAGCAAATGGAGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCTCCATTTGCTTCTGTACTCGAGTACAGAAGCAAATGGAGAATG-3'

Other clones with same target sequence:

  1. TRCN0000316500