Construct: shRNA TRCN0000108576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033605.2-1442s1c1
DNA Barcode:
CCAACAATCGAGAAGAGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dach2 (93837)
Gene Description:
dachshund 2 (Drosophila)
Transcript:
RefSeq NM_033605.2 (CURRENT)
Match location:
Position 1442 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAATCGAGAAGAGCTTA
Hairpin Sequence:
5'-CCGG-CCAACAATCGAGAAGAGCTTA-CTCGAG-TAAGCTCTTCTCGATTGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAATCGAGAAGAGCTTACTCGAGTAAGCTCTTCTCGATTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAATCGAGAAGAGCTTACTCGAGTAAGCTCTTCTCGATTGTTGG-3'

Other clones with same target sequence:

(none)