Construct: shRNA TRCN0000108671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001980.1-2392s1c1
DNA Barcode:
CCCTGAAATCTCCGTGTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Limch1 (77569)
Gene Description:
LIM and calponin homology domains 1
Transcript:
RefSeq NM_001001980.1 (NON-CURRENT)
Match location:
Position 2392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAAATCTCCGTGTCAAA
Hairpin Sequence:
5'-CCGG-CCCTGAAATCTCCGTGTCAAA-CTCGAG-TTTGACACGGAGATTTCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAAATCTCCGTGTCAAACTCGAGTTTGACACGGAGATTTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAAATCTCCGTGTCAAACTCGAGTTTGACACGGAGATTTCAGGG-3'

Other clones with same target sequence:

(none)