Construct: shRNA TRCN0000108727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177612.1-479s1c1
DNA Barcode:
GCAGTTACAAGACTCCTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctnna3 (216033)
Gene Description:
catenin (cadherin associated protein), alpha 3
Transcript:
RefSeq NM_177612.1 (NON-CURRENT)
Match location:
Position 479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTACAAGACTCCTTGTT
Hairpin Sequence:
5'-CCGG-GCAGTTACAAGACTCCTTGTT-CTCGAG-AACAAGGAGTCTTGTAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTACAAGACTCCTTGTTCTCGAGAACAAGGAGTCTTGTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTACAAGACTCCTTGTTCTCGAGAACAAGGAGTCTTGTAACTGC-3'

Other clones with same target sequence:

(none)