Construct: shRNA TRCN0000108736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013758.2-1640s1c1
DNA Barcode:
CGGGAACAGAATCGCTATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Add3 (27360)
Gene Description:
adducin 3 (gamma)
Transcript:
RefSeq NM_013758.2 (NON-CURRENT)
Match location:
Position 1640 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAACAGAATCGCTATGAT
Hairpin Sequence:
5'-CCGG-CGGGAACAGAATCGCTATGAT-CTCGAG-ATCATAGCGATTCTGTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAACAGAATCGCTATGATCTCGAGATCATAGCGATTCTGTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAACAGAATCGCTATGATCTCGAGATCATAGCGATTCTGTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000303075