Construct: shRNA TRCN0000108760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007886.1-2227s1c1
DNA Barcode:
GCATGTACTAGCATCCGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtnb (13528)
Gene Description:
dystrobrevin, beta
Transcript:
RefSeq NM_007886.1 (NON-CURRENT)
Match location:
Position 2227 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTACTAGCATCCGCATT
Hairpin Sequence:
5'-CCGG-GCATGTACTAGCATCCGCATT-CTCGAG-AATGCGGATGCTAGTACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTACTAGCATCCGCATTCTCGAGAATGCGGATGCTAGTACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTACTAGCATCCGCATTCTCGAGAATGCGGATGCTAGTACATGC-3'

Other clones with same target sequence:

(none)