Construct: shRNA TRCN0000108790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022314.2-1732s1c1
DNA Barcode:
CCACTGATTTAGCCACATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpm3 (59069)
Gene Description:
tropomyosin 3, gamma
Transcript:
RefSeq NM_022314.2 (NON-CURRENT)
Match location:
Position 1732 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGATTTAGCCACATGAA
Hairpin Sequence:
5'-CCGG-CCACTGATTTAGCCACATGAA-CTCGAG-TTCATGTGGCTAAATCAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGATTTAGCCACATGAACTCGAGTTCATGTGGCTAAATCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGATTTAGCCACATGAACTCGAGTTCATGTGGCTAAATCAGTGG-3'

Other clones with same target sequence:

(none)