Construct: shRNA TRCN0000108828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009922.2-243s1c1
DNA Barcode:
ACGGGATCATTCTTTGCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnn1 (12797)
Gene Description:
calponin 1
Transcript:
RefSeq NM_009922.2 (NON-CURRENT)
Match location:
Position 243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGGATCATTCTTTGCGAAT
Hairpin Sequence:
5'-CCGG-ACGGGATCATTCTTTGCGAAT-CTCGAG-ATTCGCAAAGAATGATCCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGGATCATTCTTTGCGAATCTCGAGATTCGCAAAGAATGATCCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGGATCATTCTTTGCGAATCTCGAGATTCGCAAAGAATGATCCCGT-3'

Other clones with same target sequence:

(none)