Construct: shRNA TRCN0000108847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011682.3-9561s1c1
DNA Barcode:
CCACTCAAGAATAGAGCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Utrn (22288)
Gene Description:
utrophin
Transcript:
RefSeq NM_011682.3 (NON-CURRENT)
Match location:
Position 9561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCAAGAATAGAGCAATA
Hairpin Sequence:
5'-CCGG-CCACTCAAGAATAGAGCAATA-CTCGAG-TATTGCTCTATTCTTGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCAAGAATAGAGCAATACTCGAGTATTGCTCTATTCTTGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCAAGAATAGAGCAATACTCGAGTATTGCTCTATTCTTGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000317441