Construct: shRNA TRCN0000108896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177771.3-331s1c1
DNA Barcode:
TGAGATTGTAATGCAGGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhl18 (270201)
Gene Description:
kelch-like 18
Transcript:
RefSeq NM_177771.3 (NON-CURRENT)
Match location:
Position 331 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGATTGTAATGCAGGGAAT
Hairpin Sequence:
5'-CCGG-TGAGATTGTAATGCAGGGAAT-CTCGAG-ATTCCCTGCATTACAATCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGATTGTAATGCAGGGAATCTCGAGATTCCCTGCATTACAATCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGATTGTAATGCAGGGAATCTCGAGATTCCCTGCATTACAATCTCA-3'

Other clones with same target sequence:

(none)