Construct: shRNA TRCN0000108907

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018790.1-676s1c1
DNA Barcode:
GATGGCTATGACTATACCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arc (11838)
Gene Description:
activity regulated cytoskeletal-associated protein
Transcript:
RefSeq NM_018790.1 (NON-CURRENT)
Match location:
Position 676 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108907)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGCTATGACTATACCGTT
Hairpin Sequence:
5'-CCGG-GATGGCTATGACTATACCGTT-CTCGAG-AACGGTATAGTCATAGCCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGCTATGACTATACCGTTCTCGAGAACGGTATAGTCATAGCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGCTATGACTATACCGTTCTCGAGAACGGTATAGTCATAGCCATC-3'

Other clones with same target sequence:

(none)