Construct: shRNA TRCN0000108925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007984.1-1593s1c1
DNA Barcode:
CCTCCTGTTATCCTTACTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fscn1 (14086)
Gene Description:
fascin actin-bundling protein 1
Transcript:
RefSeq NM_007984.1 (NON-CURRENT)
Match location:
Position 1593 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTGTTATCCTTACTCAT
Hairpin Sequence:
5'-CCGG-CCTCCTGTTATCCTTACTCAT-CTCGAG-ATGAGTAAGGATAACAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTGTTATCCTTACTCATCTCGAGATGAGTAAGGATAACAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTGTTATCCTTACTCATCTCGAGATGAGTAAGGATAACAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000335364