Construct: shRNA TRCN0000108929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007984.1-1449s1c1
DNA Barcode:
CTACAATAAGGTGGCTCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fscn1 (14086)
Gene Description:
fascin actin-bundling protein 1
Transcript:
RefSeq NM_007984.1 (NON-CURRENT)
Match location:
Position 1449 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACAATAAGGTGGCTCTCAA
Hairpin Sequence:
5'-CCGG-CTACAATAAGGTGGCTCTCAA-CTCGAG-TTGAGAGCCACCTTATTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAATAAGGTGGCTCTCAACTCGAGTTGAGAGCCACCTTATTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAATAAGGTGGCTCTCAACTCGAGTTGAGAGCCACCTTATTGTAG-3'

Other clones with same target sequence:

  1. TRCN0000363835