Construct: shRNA TRCN0000108962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024184.1-246s1c1
DNA Barcode:
CCTGGAGTGGAAGATCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asf1b (66929)
Gene Description:
anti-silencing function 1B histone chaperone
Transcript:
RefSeq NM_024184.1 (NON-CURRENT)
Match location:
Position 246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAGTGGAAGATCATTTA
Hairpin Sequence:
5'-CCGG-CCTGGAGTGGAAGATCATTTA-CTCGAG-TAAATGATCTTCCACTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAGTGGAAGATCATTTACTCGAGTAAATGATCTTCCACTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAGTGGAAGATCATTTACTCGAGTAAATGATCTTCCACTCCAGG-3'

Other clones with same target sequence:

(none)