Construct: shRNA TRCN0000108965
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_018774.1-3377s1c1
- DNA Barcode:
- CCTCCAGATAGCAAGAGGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Phc2 (54383)
- Gene Description:
- polyhomeotic-like 2 (Drosophila)
- Transcript:
- RefSeq NM_018774.1 (NON-CURRENT)
- Match location:
- Position 3377 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000108965)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCCAGATAGCAAGAGGTTT
- Hairpin Sequence:
-
5'-CCGG-CCTCCAGATAGCAAGAGGTTT-CTCGAG-AAACCTCTTGCTATCTGGAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCCAGATAGCAAGAGGTTTCTCGAGAAACCTCTTGCTATCTGGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCCAGATAGCAAGAGGTTTCTCGAGAAACCTCTTGCTATCTGGAGG-3'
Other clones with same target sequence:
(none)
