Construct: shRNA TRCN0000108983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172663.2-2517s1c1
DNA Barcode:
CCTGTCAATGTGCATATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epc2 (227867)
Gene Description:
enhancer of polycomb homolog 2 (Drosophila)
Transcript:
RefSeq NM_172663.2 (NON-CURRENT)
Match location:
Position 2517 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000108983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCAATGTGCATATCAAT
Hairpin Sequence:
5'-CCGG-CCTGTCAATGTGCATATCAAT-CTCGAG-ATTGATATGCACATTGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCAATGTGCATATCAATCTCGAGATTGATATGCACATTGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCAATGTGCATATCAATCTCGAGATTGATATGCACATTGACAGG-3'

Other clones with same target sequence:

(none)