Construct: shRNA TRCN0000109007

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007790.2-1895s1c1
DNA Barcode:
CCTGAAACTAACGATGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smc3 (13006)
Gene Description:
structural maintenance of chromosomes 3
Transcript:
RefSeq NM_007790.2 (NON-CURRENT)
Match location:
Position 1895 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109007)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAACTAACGATGCTATT
Hairpin Sequence:
5'-CCGG-CCTGAAACTAACGATGCTATT-CTCGAG-AATAGCATCGTTAGTTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAACTAACGATGCTATTCTCGAGAATAGCATCGTTAGTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAACTAACGATGCTATTCTCGAGAATAGCATCGTTAGTTTCAGG-3'

Other clones with same target sequence:

(none)