Construct: shRNA TRCN0000109015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009282.2-5724s1c1
DNA Barcode:
CCCTGGAATAAGCACAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stag1 (20842)
Gene Description:
stromal antigen 1
Transcript:
RefSeq NM_009282.2 (NON-CURRENT)
Match location:
Position 5724 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGAATAAGCACAGATAA
Hairpin Sequence:
5'-CCGG-CCCTGGAATAAGCACAGATAA-CTCGAG-TTATCTGTGCTTATTCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGAATAAGCACAGATAACTCGAGTTATCTGTGCTTATTCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGAATAAGCACAGATAACTCGAGTTATCTGTGCTTATTCCAGGG-3'

Other clones with same target sequence:

(none)