Construct: shRNA TRCN0000109138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198102.1-870s1c1
DNA Barcode:
CGAGGCTATGACAGATACGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tra2a (101214)
Gene Description:
transformer 2 alpha homolog (Drosophila)
Transcript:
RefSeq NM_198102.1 (NON-CURRENT)
Match location:
Position 870 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGCTATGACAGATACGAA
Hairpin Sequence:
5'-CCGG-CGAGGCTATGACAGATACGAA-CTCGAG-TTCGTATCTGTCATAGCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGCTATGACAGATACGAACTCGAGTTCGTATCTGTCATAGCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGCTATGACAGATACGAACTCGAGTTCGTATCTGTCATAGCCTCG-3'

Other clones with same target sequence:

(none)